Journal: Frontiers in Immunology

Article Title: Targeting the oxidative stress-neuroinflammation axis: the mechanism of arctigenin’s broad-spectrum analgesia with limited side effects

doi: 10.3389/fimmu.2026.1754756

Figure Lengend Snippet: The antiallodynic effects of AG in SNI mice are associated with MAPK signaling pathway. (A) A shows western blotting of p-ERK and t-ERK in each group of spinal cord and qualitative data of protein expression in different groups. (B) Western blotting of p-JNK and t-JNK in different groups. (C) Western blotting of p-p38 and t-p38 in different groups. (D) Western blotting of p-mTOR in different groups. (E) Western blotting of PGC-a in different groups. (F) Western blotting of p-AMPK in different groups. (G) The analgesic effects of AG in SNI mice could be blocked by pretreatment with inhibitors of the MAPK pathway [U0126 (100 μg/kg, i.p.), SB203580 (40 μg/ml, 10 ml/kg, i.p.), or SP600125 (50 μg/kg, i.p.)], as measured by von Frey testing. (H) The extent and duration of analgesia are estimated by the area under curve (AUC (g min)) of PWT vs time (0-120 minutes). Data are expressed as the mean ± SEM. ** p < 0.01 and *** p < 0.001 for sham group vs SNI group. # p < 0.05, and ## p < 0.01 for SNI group vs AG+SNI group. && p < 0.01 compared with AG+SNI group. The "ns" indicates no significant difference.

Article Snippet: U0126 (inhibitor of ERK, cat number: HY-12031A), SB20358 (inhibitor of p38, cat number: HY-112349) and SP600125 (inhibitor of JNK, cat number: HY-12041) were purchased from MedChemExpress.

Techniques: Western Blot, Expressing

Journal: Poultry Science

Article Title: Novel insights into the regulation of NF2/SWH signaling in granulosa cells of prehierarchical follicle development in hen ovary

doi: 10.1016/j.psj.2026.106375

Figure Lengend Snippet: Effect of NF2/SWH signaling pathway on GC apoptosis via p38 signaling in chicken ovary. GCs were transfected with specific siRNA-NF2 and/or siRNA-YAP1, NC (scrambled siRNA, negative control), and no siRNA as a vehicle (blank control, BC), U46619, and SB203580. (A-B) The p-p38, p38 protein expression level in the GCs was analyzed using western blot. (C) Western blot analysis was employed to screen out the best processing time of U46619 (an activator of p38 MAPK phosphorylation) and SB203580 (an inhibitor of p38 MAPK phosphorylation). (D) Effects of different treatments on GC apoptosis. Cell apoptosis rate was measured through flow cytometry. (E) Expression of BCL2 and CASP3 mRNA and proteins in the GCs with different treatments were analyzed by RT-qPCR and western blot. Experimental procedures were performed in triplicate, and representative results report both means and standard deviations. For each group, the different superscript above the bar indicates that the difference was significant (* P < 0.05, ** P < 0.01).

Article Snippet: GCs that had been cultured for 24 h were washed twice with PBS and were then preincubated with or without 10 μM SB203580 (an inhibitor of p38 MAPK phosphorylation) or U46619 (an activator of p38 MAPK phosphorylation) for 1h and 2 h to screen out the best processing time of SB203580 (S1863, Beyotime, Jiangsu, China) and U46619 (sc-201242, Santa Cruz, Dallas, TX, USA) as described previously ( , , , ).

Techniques: Transfection, Negative Control, Control, Expressing, Western Blot, Phospho-proteomics, Flow Cytometry, Quantitative RT-PCR

Journal: Poultry Science

Article Title: Novel insights into the regulation of NF2/SWH signaling in granulosa cells of prehierarchical follicle development in hen ovary

doi: 10.1016/j.psj.2026.106375

Figure Lengend Snippet: Effect of NF2/SWH signaling pathway on GC mitochondria via p38 signaling. GCs were transfected with specific siRNA-NF2 and/or siRNA-YAP1, NC (scrambled siRNA, negative control), and no siRNA as a vehicle (blank control, BC), U46619, and SB203580. (A) Effects of different treatments on mitochondrial gene DNA copy number. (B) Effects of different treatments on ATP. (C) Effects of different treatments on cytochrome C. (D) Effects of different treatments on ROS. (E) Effects of different treatments on the membrane potential. Experimental procedures were performed in triplicate, and representative results report both means and standard deviations. For each group, the different superscript above the bar indicates that the difference was significant (* P < 0.05).

Article Snippet: GCs that had been cultured for 24 h were washed twice with PBS and were then preincubated with or without 10 μM SB203580 (an inhibitor of p38 MAPK phosphorylation) or U46619 (an activator of p38 MAPK phosphorylation) for 1h and 2 h to screen out the best processing time of SB203580 (S1863, Beyotime, Jiangsu, China) and U46619 (sc-201242, Santa Cruz, Dallas, TX, USA) as described previously ( , , , ).

Techniques: Transfection, Negative Control, Control, Membrane